Application of click chemistry to the production of DNA microarrays.
نویسندگان
چکیده
The copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction was applied as the novel method of DNA immobilization on a modified solid support. The CuAAC click reaction enables the covalent binding of DNA modified with pentynyl groups at its 5'-end to azide-loaded slides. Click microarrays were produced using this approach and successfully employed in biological/model experiments.
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ورودعنوان ژورنال:
- Lab on a chip
دوره 12 6 شماره
صفحات -
تاریخ انتشار 2012